Changing sample protein identification page 3 a check mark will appear next to the injection in the experiment pane to indicate a manual correction was made. Fluorchem fc3 motorized lens upgrade for alphaimager hp for chemiluminescence applications. Faster and easier charge heterogeneity analysis with the ice 3. The ice3 system performs free solution ief in a capillary column cief and detects focused protein zones using a whole column uv absorption detector that avoids disturbing these focused zones. As an added bonus, you can use the same generic method for multiple moleculesno need for productspecific methods. To support the use of ice3 in qc and gmp environments, all required functionality is integrated in ice cfr software to ensure compliance with 21 cfr part 11. Before you do anything else, please take a look in the box and check you have the items shown below. Jess chemiluminescent and fluorescent western blotting. Specialized in phosphoprotein signaling, protein purity, protein aggregation, protein fractionation, western blot imaging, gel documentation.
Mfi bot1 autosampler reagents and consumables are designed for use on the bot1 autosampler when it is attached to the mfi 5100 or mfi 5200 instrument. User manuals, icematic ice maker operating guides and service manuals. Maurices native fluorescence detection for cief works by measuring the fluorescence emission of tryptophans aromatic group. Biorad gs800 calibrated densitometer user manual calibration tablet areascalibration windows the gs800 densitometer is equipped with reflectance and transmission step wedges. Protein simple ice3 user manual keyword after analyzing the system lists the list of keywords related and the list of websites with related content, in addition you can see which keywords most interested customers on the this website. We develop and commercialise proprietary systems and consumables for protein analysis that ultimately help reveal new insights into the true nature of proteins. Jess gives you four different ways to analyze proteins. Your data is reliable no matter whatacross samples, users, instruments or labs. The ice manuals series is the single most comprehensive collection of civil engineering knowledge available, covering key subject areas of civil engineering. Simple western which protein is present and how much. Handset description handset description front view back view earpiece mirror camera speaker 65,000 colour main display left soft key right soft key. Users guide to protein and amino acids basic health. Both ice280 and ice3 use protein absorption at 280 nm to monitor charge heterogeneity, and now maurice adds native fluorescence detection to greatly increase cief capabilities. Ella helps the user to get fast and highly reproducible validated assay data with no manual steps.
It offers single plex, multiplex, multianalyte and customizable possibilities. Fluorchem fc3 manual lens upgrade for alphaimager hp for chemiluminescence applications. Imaged capillary ief is quickly becoming the method of choice for many biopharmaceutical companies. Select the ice function under digital ice 3 from the toolbar menu at the top of the cyberview x screen to enable or disable the ice function. User manual instruction guides for proteinsimple devices.
User manual instruction guide for protein detection instrument maurice proteinsimple. Regulatory agencies routinely require assessment of charge heterogeneity for biotechnology products. The maurice cief method development kit is a onestop. Proteinsimple protein detection instrument maurice manual. This technology is unique in that it has the comparable resolution of traditional gel ief but incorporates the advantages. Maurice, ice3, and ice280 data equivalency for cief charge heterogeneity. Safety datasheets product inserts user guides service contracts ice cfr software. Proteinsimple ice280 ice systems first wholecolumn. Automatically restores original colors from the faded films. Variability in the manual processes that used to impact reproducibility, quantitation, time to result and overall data reliability is eliminated. For information on flow cell options, please refer to the mfi 5000 series reagents and consumables section. Biotechne warrants that the instrument purchased from biotechne the instrument will be free from defects in materials and workmanship during the applicable warranty period, provided that the instrument has been operated at all times in accordance with the instruction manual and user guide by or under the direct supervision of a certified. Proteinsimple polypropylene vial with 300ul manufacturer. This user s guide demystifies protein and amino acids and explains how readers can use them to enhance their health.
Maurice systems take cief up a notch, and cesds application is a breeze. User manual instruction guide for proteinsimple devices proteinsimple user manuals proteinsimple. The technique is useful for separation of glycoforms. Maurice, ice3, and ice280 data equivalency for cief charge heterogeneity absorbance assays.
Our goal is to make protein analysis simpler, more quantitative and affordable. Use the email address on your account instead of your user id to sign in with your current password. Proteinsimple maurice protein detection instrument user. Perfect for all engineers seeking guidance on the principles of major civil engineering subject areas those in the first years of practice, the more experienced. Japanese using the simple western total protein assay to normalize immunoassay data in the same run. Proteinsimple 0453 polypropylene vial with 300ul molded insert, 100pkg. Biologics ice3, maurice and mfi is my biologic drug pure. Secrets of ice method design for protein therapeutics. System for analysis of protein heterogeneity protein simple ice3. Our goal is simply to help researchers gain a better understanding of proteins and their role in disease. Characterization of the protein solubilizer simplesol for imaged capillary.
Simple plex which biomarkers are present and how much. Suitable for use with the ice3 alcott 720 and ice280alcott 719 only. Protein simple ice3 user manual keyword found websites. Since 2001, scott has held various posts, focusing on assay development for the fields of cellular. Proteinsimple maurice protein detection instrument user manual maurice manual. Mfi 5200 particle analyzer from proteinsimple biocompare. Icematic ice maker user manuals download manualslib. Click roc function under digital ice3 menu, roc setting window will open up. Protein charge heterogeneity analysis in 10 minutes.
Run multiple characterizations from a single system that biopharma labs depend on, and produce qualitative and quantitative analyses, with speed and confidence. The maurice cief method development kit is a one stop. No messy gels, no transfer tanks, no blots, and no manual analysis. Simply load up to 96 samples, push a button, walk away and come back to fully analyzed data. Protein consists of amino acids, which are used in the construction of neurotransmitters, hormones, muscle and other tissues. Maurice protein detection instrument user manual maurice. Protein ice blue raspberry 12 drinks by ansi nutrition. Scott mack is a senior development scientist with proteinsimple currently supporting analysts utilizing imaged capillary electrophoresis ice, simple western and microflow imaging mfi technology for the characterization of protein therapeutics. Get better overall workflow and time to result with ice3 and maurice theyre fluent in cief and cesds. The ice280 was the first to do freesolution ief in a capillary column cief, detect focused protein zones using a whole column uv absorption detector, and not disturb those f.
With the pa 800 plus pharmaceutical analysis system, you can confidently safeguard the success of your biologics. Proteinsimple provides protein analysis tools to help researchers gain a better understanding of proteins and their role in disease. The simple western is a western with none of the hassle. Proteinsimple ice280 first wholecolumn cief system by proteinsimple. Now, with the latest compass software, researchers can easily validate their simple western work to ensure absolute data integrity. Capillary isoelectric focusing of a difficulttodenature. Setup instructions, pairing guide, and how to reset. Capillary isoelectric focusing cief 1 is a useful technique for the determination of protein isoelectric point pi. Cartridge installation and removal on ice3 proteinsimple. Get better overall workflow and time to result with ice3 and maurice. Best of all, the transition is simple the same antibodies you use for western blotting protocols can be used with simple western assays.
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